Fluorescent ratiometric MFC probe sensitive to early stages of alpha-synuclein aggregation.
نویسندگان
چکیده
We introduce a sensor molecule, AS140-MFC, consisting of a covalent adduct of an Ala-to-Cys mutant of alpha-synuclein with the 3-hydroxychromone dual emission dye MFC. We show that the AS140-MFC construct is a multiparametric fluorescent probe suitable for the continuous monitoring of protein aggregation and is sensitive to the early and intermediate stages of alpha-synuclein aggregation, a process associated with Parkinson's disease.
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ورودعنوان ژورنال:
- Journal of the American Chemical Society
دوره 132 23 شماره
صفحات -
تاریخ انتشار 2010